Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica

Latest version published by SCAR - Microbial Antarctic Resource System on Jan 16, 2020 SCAR - Microbial Antarctic Resource System

This dataset describes heterotrophic bacteria that were isolated from five aquatic microbial mat samples from different locations in continental Antarctica and the Antarctic Peninsula.

Data Records

The data in this sampling event resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 9 records. 1 extension data tables also exist. An extension record supplies extra information about a core record. The number of records in each extension data table is illustrated below.

  • Event (core)
    9
  • Occurrence 
    504

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Downloads

Download the latest version of this resource data as a Darwin Core Archive (DwC-A) or the resource metadata as EML or RTF:

Data as a DwC-A file download 9 records in English (14 KB) - Update frequency: unknown
Metadata as an EML file download in English (9 KB)
Metadata as an RTF file download in English (9 KB)

Versions

The table below shows only published versions of the resource that are publicly accessible.

How to cite

Researchers should cite this work as follows:

Peeters K (2020): Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Samplingevent. https://ipt.biodiversity.aq/resource?r=heterotrophic_bacterial_isolates_in_antarctica_microbial_mats&v=1.0

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 74277118-41a6-4e2b-bfe3-4d002cca369f.  SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.

Keywords

Samplingevent

Contacts

Who created the resource:

Karolien Peeters
Ghent University Ghent BE

Who can answer questions about the resource:

Anne Willems
Ghent University Ghent BE

Who filled in the metadata:

Maxime Sweetlove
Royal Belgian Institute of Natural Sciences Rue Vautier 29 1000 Brussels

Who else was associated with the resource:

User
Anne Willems
Ghent University Ghent BE
Karolien Peeters
Ghent University Ghent BE

Geographic Coverage

Different water bodies (lakes) on the Antarctic continent: East Antarctica, the Trans-Antarctic Mountains, and the Antarctic Peninsula

Bounding Coordinates South West [-82.45, -67.45], North East [-67.7, 39.8]

Taxonomic Coverage

Heterotrophic Bacteria (504 strains) isolated from frozen microbial mat samples using culturing techniques.

Kingdom  Bacteria (Bacteria)

Project Data

The AMBIO project aimed to extend the baseline information of microbial diversity in aquatic habitats of terrestrial Antarctic environments.

Title Antarctic Microbial Biodiversity (AMBIO)
Funding BelSPO SD/BA/01
Study Area Description the Antarctic continent.

The personnel involved in the project:

Annick Wilmotte
Anne Willems
Elie Verleyen
Wim Vyverman

Sampling Methods

Microbial mat samples were collected aseptically using a sterile spatula, and were kept frozen continuously after collection until processing in the laboratory.

Study Extent Bacterial strain isolated from five aquatic microbial mat samples from different locations in continental Antarctica and the Antarctic Peninsula, sampled between 2003 and 2007.

Method step description:

  1. One gram of sample was aseptically weighed and homogenized in 9 ml sterile cold (4°C) physiological saline (0.86% NaCl) using a vortex. Tenfold dilution series (kept at 4°C) were plated on four different media (Marine agar 2216 (MA) (BD DifcoTM), R2A (BD DifcoTM), ten times diluted R2A (R2A/10), and PYGV (Pepton-Yeast-Glucose-Vitamin) medium (DSMZ medium 621)) and incubated at 20°C, 15°C and 4°C. R2A (Difco) contains pyruvate, starch and dextrose as C sources and yeast extract, peptone and casaminoacids as N and C sources and PYGV (DSMZ medium 621) contains peptone, yeast extract and glucose as C and/or N sources and additional vitamins and minerals. Both are considered oligotrophic media because the amounts of these components are at least two to ten times lower than in more general media such as nutrient broth. In addition to regular physiological saline (PS) dilution series, sea water (SW) dilutions were used for the LA3 and WO10 samples which originated from lakes close to the ocean and had elevated conductivity values.

Collection Data

Collection Name BCCM-LMG
Collection Identifier BCCM-LMG

Bibliographic Citations

  1. K. Peeters, E. Verleyen, D. A. Hodgson, P. Convey, D. Ertz , W. Vyverman, A. Willems (2012). Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica. Polar Biol (2012) 35:543‐554. DOI 10.1007/s00300‐011‐1100‐4