Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica

Data Records

The data in this sampling event resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 9 records.

1 extension data tables also exist. An extension record supplies extra information about a core record. The number of records in each extension data table is illustrated below.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versions

The table below shows only published versions of the resource that are publicly accessible.

How to cite

Researchers should cite this work as follows:

Peeters K (2020): Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Samplingevent. https://ipt.biodiversity.aq/resource?r=heterotrophic_bacterial_isolates_in_antarctica_microbial_mats&v=1.0

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 74277118-41a6-4e2b-bfe3-4d002cca369f.  SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.

Keywords

Samplingevent

Contacts

Geographic Coverage

Different water bodies (lakes) on the Antarctic continent: East Antarctica, the Trans-Antarctic Mountains, and the Antarctic Peninsula

Taxonomic Coverage

Heterotrophic Bacteria (504 strains) isolated from frozen microbial mat samples using culturing techniques.

Project Data

The AMBIO project aimed to extend the baseline information of microbial diversity in aquatic habitats of terrestrial Antarctic environments.

The personnel involved in the project:

Sampling Methods

Microbial mat samples were collected aseptically using a sterile spatula, and were kept frozen continuously after collection until processing in the laboratory.

Method step description:

1. One gram of sample was aseptically weighed and homogenized in 9 ml sterile cold (4°C) physiological saline (0.86% NaCl) using a vortex. Tenfold dilution series (kept at 4°C) were plated on four different media (Marine agar 2216 (MA) (BD DifcoTM), R2A (BD DifcoTM), ten times diluted R2A (R2A/10), and PYGV (Pepton-Yeast-Glucose-Vitamin) medium (DSMZ medium 621)) and incubated at 20°C, 15°C and 4°C. R2A (Difco) contains pyruvate, starch and dextrose as C sources and yeast extract, peptone and casaminoacids as N and C sources and PYGV (DSMZ medium 621) contains peptone, yeast extract and glucose as C and/or N sources and additional vitamins and minerals. Both are considered oligotrophic media because the amounts of these components are at least two to ten times lower than in more general media such as nutrient broth. In addition to regular physiological saline (PS) dilution series, sea water (SW) dilutions were used for the LA3 and WO10 samples which originated from lakes close to the ocean and had elevated conductivity values.

Collection Data

Bibliographic Citations

1. K. Peeters, E. Verleyen, D. A. Hodgson, P. Convey, D. Ertz , W. Vyverman, A. Willems (2012). Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica. Polar Biol (2012) 35:543‐554. DOI 10.1007/s00300‐011‐1100‐4

Additional Metadata