Microbial communities in colored snow from Fildes Peninsula (King George Island, Maritime Antarctica, 2017)

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Versão mais recente published by SCAR - Microbial Antarctic Resource System on jun 18, 2021 SCAR - Microbial Antarctic Resource System
Publication date:
18 de junho de 2021
Licença:
CC-BY 4.0

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Descrição

Amplicon sequencing dataset (Illumina MiSeq) targeting Eukaryota (18S ssu rRNA) and Bacteria (16S ssu rRNA) in red or green colored snow samples (n=10) from Fildes Peninsula, Antarctica (2017).

Versões

A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.

Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Luo W, Ding H, Li H, Ji Z, Huang K, Zhao W, Yu Y, Zeng Y (2021): Microbial communities in colored snow from Fildes Peninsula (King George Island, Maritime Antarctica, 2017). v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbial_communities_colored_snow_antarctica_2017&v=1.0

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: de1c2235-6bf9-4443-bb5c-e0f0feb8ca77.  SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.

Palavras-chave

Metadata

Contatos

Wei Luo
  • Originador ●
  • Usuário ●
  • Ponto De Contato
Polar Research Institute of China
Shangai
CN
Haitao Ding
  • Originador
Polar Research Institute of China
Shangai
CN
Huirong Li
  • Originador
Polar Research Institute of China
Shangai
CN
Zhongqiang Ji
  • Originador
Second Institute of Oceanography
Hangzhou
CN
Kaiyao Huang
  • Originador
Chinese Academy of Sciences
Wuhan
CN
Wenyu Zhao
  • Originador
CN
Yong Yu
  • Originador
Polar Research Institute of China
Shangai
CN
Yinxin Zeng
  • Originador
Polar Research Institute of China
Shangai
CN
Maxime Sweetlove
  • Provedor Dos Metadados
Royal Belgian Institute of Natural Sciences
Brussels
BE

Cobertura Geográfica

Antarctica:Fildes Peninsula

Coordenadas delimitadoras Sul Oeste [-62,206, -58,971], Norte Leste [-62,191, -58,964]

Cobertura Temporal

Data Inicial 2017-01-30

Dados Sobre o Projeto

Nenhuma descrição disponível

Título Microbial communities in colored snow from Fildes Peninsula (King George Island, Maritime Antarctica, 2017)
Financiamento This research was supported by the National Natural Science Foundation of China (No. 91851201; No. 41376191), the National Key R&D Program of China (No: 2018YFC1406903), the State Key Laboratory of Microbial Metabolism (Shanghai Jiao Tong University, China: MMLKF16-10), and the Key Research and Development Program of Science and Technology Innovation Project of Hunan Province (2018SK2011).

O pessoal envolvido no projeto:

Wei Luo

Métodos de Amostragem

Samples were taken aseptically, using a 30 × 30 cm colored snow squares for each sampling station.

Área de Estudo Colored snow samples (500 mL) from Fildes Peninsula (Antarctica) taken on 2017-01-30.

Descrição dos passos do método:

  1. Samples were allowed to naturally melt (≤ 10 °C) and were subsequently filtered through a 0.2 μm nucleopore membrane filter (Whatman). The filters were frozen at − 80 °C in cetyltrimethyl ammonium bromide (CTAB) buffer until analysis. DNA extraction was performed as described by Luo et al. (2017). PCR of microbial eukaryotes targeted the V4 region of the 18S ribosomal RNA (rRNA) gene: forward primer 3NDf (5ʹ-GGCAAGTCTGGTGCCAG-3ʹ) and reverse primer V4_euk_R2 (5ʹ-ACGGTATCTRATCRTCTTCG-3ʹ). The V4–V5 hypervariable regions of the bacte- rial 16S rRNA gene were amplified using PCR with the universal primers 515F-Y (5ʹ-GTGYCAGCMGCCGCG GTAA-3ʹ) and 926R (5ʹ-CCGYCAATTYMTTTRAG TTT-3ʹ).
  2. PCR products were pooled and purified using a DNA gel extraction kit (Axygen, Hangzhou, China). The DNA concentration was determined using a Quant-iT PicoGreen double-stranded DNA assay (Invitrogen, Germany), and quality was examined with a TBS-380 Mini-Fluorometer (Turner Biosystems, Sunnyvale, CA, USA). Finally, amplicons of all samples were pooled in equimolar concentrations and sequenced on an Illumina MiSeq2000 platform.

Citações bibliográficas

  1. Luo, W., Ding, H., Li, H., Ji, Z., Huang, K., Zhao, W., ... & Zeng, Y. (2020). Molecular diversity of the microbial community in coloured snow from the Fildes Peninsula (King George Island, Maritime Antarctica). Polar Biology, 43(9), 1391-1405.