Microbial communities in colored snow from Fildes Peninsula (King George Island, Maritime Antarctica, 2017)

元數據
最新版本 published by SCAR - Microbial Antarctic Resource System on 6月 18, 2021 SCAR - Microbial Antarctic Resource System
發布日期:
2021年6月18日
授權條款:
CC-BY 4.0

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說明

Amplicon sequencing dataset (Illumina MiSeq) targeting Eukaryota (18S ssu rRNA) and Bacteria (16S ssu rRNA) in red or green colored snow samples (n=10) from Fildes Peninsula, Antarctica (2017).

版本

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如何引用

研究者應依照以下指示引用此資源。:

Luo W, Ding H, Li H, Ji Z, Huang K, Zhao W, Yu Y, Zeng Y (2021): Microbial communities in colored snow from Fildes Peninsula (King George Island, Maritime Antarctica, 2017). v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbial_communities_colored_snow_antarctica_2017&v=1.0

權利

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此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF 註冊

此資源已向GBIF註冊，並指定以下之GBIF UUID: de1c2235-6bf9-4443-bb5c-e0f0feb8ca77。  SCAR - Microbial Antarctic Resource System 發佈此資源，並經由Scientific Committee on Antarctic Research同意向GBIF註冊成為資料發佈者。

關鍵字

Metadata

聯絡資訊

Wei Luo
  • 出處 ●
  • 使用者 ●
  • 連絡人
Polar Research Institute of China
Shangai
CN
Haitao Ding
  • 出處
Polar Research Institute of China
Shangai
CN
Huirong Li
  • 出處
Polar Research Institute of China
Shangai
CN
Zhongqiang Ji
  • 出處
Second Institute of Oceanography
Hangzhou
CN
Kaiyao Huang
  • 出處
Chinese Academy of Sciences
Wuhan
CN
Wenyu Zhao
  • 出處
CN
Yong Yu
  • 出處
Polar Research Institute of China
Shangai
CN
Yinxin Zeng
  • 出處
Polar Research Institute of China
Shangai
CN
Maxime Sweetlove
  • 元數據提供者
Royal Belgian Institute of Natural Sciences
Brussels
BE

地理涵蓋範圍

Antarctica:Fildes Peninsula

界定座標範圍 緯度南界 經度西界 [-62.206, -58.971], 緯度北界 經度東界 [-62.191, -58.964]

時間涵蓋範圍

起始日期 2017-01-30

計畫資料

無相關描述

計畫名稱 Microbial communities in colored snow from Fildes Peninsula (King George Island, Maritime Antarctica, 2017)
經費來源 This research was supported by the National Natural Science Foundation of China (No. 91851201; No. 41376191), the National Key R&D Program of China (No: 2018YFC1406903), the State Key Laboratory of Microbial Metabolism (Shanghai Jiao Tong University, China: MMLKF16-10), and the Key Research and Development Program of Science and Technology Innovation Project of Hunan Province (2018SK2011).

參與計畫的人員:

Wei Luo

取樣方法

Samples were taken aseptically, using a 30 × 30 cm colored snow squares for each sampling station.

研究範圍 Colored snow samples (500 mL) from Fildes Peninsula (Antarctica) taken on 2017-01-30.

方法步驟描述:

  1. Samples were allowed to naturally melt (≤ 10 °C) and were subsequently filtered through a 0.2 μm nucleopore membrane filter (Whatman). The filters were frozen at − 80 °C in cetyltrimethyl ammonium bromide (CTAB) buffer until analysis. DNA extraction was performed as described by Luo et al. (2017). PCR of microbial eukaryotes targeted the V4 region of the 18S ribosomal RNA (rRNA) gene: forward primer 3NDf (5ʹ-GGCAAGTCTGGTGCCAG-3ʹ) and reverse primer V4_euk_R2 (5ʹ-ACGGTATCTRATCRTCTTCG-3ʹ). The V4–V5 hypervariable regions of the bacte- rial 16S rRNA gene were amplified using PCR with the universal primers 515F-Y (5ʹ-GTGYCAGCMGCCGCG GTAA-3ʹ) and 926R (5ʹ-CCGYCAATTYMTTTRAG TTT-3ʹ).
  2. PCR products were pooled and purified using a DNA gel extraction kit (Axygen, Hangzhou, China). The DNA concentration was determined using a Quant-iT PicoGreen double-stranded DNA assay (Invitrogen, Germany), and quality was examined with a TBS-380 Mini-Fluorometer (Turner Biosystems, Sunnyvale, CA, USA). Finally, amplicons of all samples were pooled in equimolar concentrations and sequenced on an Illumina MiSeq2000 platform.

引用文獻

  1. Luo, W., Ding, H., Li, H., Ji, Z., Huang, K., Zhao, W., ... & Zeng, Y. (2020). Molecular diversity of the microbial community in coloured snow from the Fildes Peninsula (King George Island, Maritime Antarctica). Polar Biology, 43(9), 1391-1405.