Microbial communities in colored snow from Fildes Peninsula (King George Island, Maritime Antarctica, 2017)

Latest version published by SCAR - Microbial Antarctic Resource System on Jun 18, 2021 SCAR - Microbial Antarctic Resource System

Amplicon sequencing dataset (Illumina MiSeq) targeting Eukaryota (18S ssu rRNA) and Bacteria (16S ssu rRNA) in red or green colored snow samples (n=10) from Fildes Peninsula, Antarctica (2017).

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How to cite

Researchers should cite this work as follows:

Luo W, Ding H, Li H, Ji Z, Huang K, Zhao W, Yu Y, Zeng Y (2021): Microbial communities in colored snow from Fildes Peninsula (King George Island, Maritime Antarctica, 2017). v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbial_communities_colored_snow_antarctica_2017&v=1.0

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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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This resource has been registered with GBIF, and assigned the following GBIF UUID: de1c2235-6bf9-4443-bb5c-e0f0feb8ca77.  SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.

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Metadata

Contacts

Who created the resource:

Wei Luo
Polar Research Institute of China Shangai CN
Haitao Ding
Polar Research Institute of China Shangai CN
Huirong Li
Polar Research Institute of China Shangai CN
Zhongqiang Ji
Second Institute of Oceanography Hangzhou CN
Kaiyao Huang
Chinese Academy of Sciences Wuhan CN
Wenyu Zhao
CN
Yong Yu
Polar Research Institute of China Shangai CN
Yinxin Zeng
Polar Research Institute of China Shangai CN

Who can answer questions about the resource:

Wei Luo
Polar Research Institute of China Shangai CN

Who filled in the metadata:

Maxime Sweetlove
Royal Belgian Institute of Natural Sciences Brussels BE

Who else was associated with the resource:

User
Wei Luo
Polar Research Institute of China Shangai CN

Geographic Coverage

Antarctica:Fildes Peninsula

Bounding Coordinates South West [-62.206, -58.971], North East [-62.191, -58.964]

Temporal Coverage

Start Date 2017-01-30

Project Data

No Description available

Title Microbial communities in colored snow from Fildes Peninsula (King George Island, Maritime Antarctica, 2017)
Funding This research was supported by the National Natural Science Foundation of China (No. 91851201; No. 41376191), the National Key R&D Program of China (No: 2018YFC1406903), the State Key Laboratory of Microbial Metabolism (Shanghai Jiao Tong University, China: MMLKF16-10), and the Key Research and Development Program of Science and Technology Innovation Project of Hunan Province (2018SK2011).

The personnel involved in the project:

Wei Luo

Sampling Methods

Samples were taken aseptically, using a 30 × 30 cm colored snow squares for each sampling station.

Study Extent Colored snow samples (500 mL) from Fildes Peninsula (Antarctica) taken on 2017-01-30.

Method step description:

  1. Samples were allowed to naturally melt (≤ 10 °C) and were subsequently filtered through a 0.2 μm nucleopore membrane filter (Whatman). The filters were frozen at − 80 °C in cetyltrimethyl ammonium bromide (CTAB) buffer until analysis. DNA extraction was performed as described by Luo et al. (2017). PCR of microbial eukaryotes targeted the V4 region of the 18S ribosomal RNA (rRNA) gene: forward primer 3NDf (5ʹ-GGCAAGTCTGGTGCCAG-3ʹ) and reverse primer V4_euk_R2 (5ʹ-ACGGTATCTRATCRTCTTCG-3ʹ). The V4–V5 hypervariable regions of the bacte- rial 16S rRNA gene were amplified using PCR with the universal primers 515F-Y (5ʹ-GTGYCAGCMGCCGCG GTAA-3ʹ) and 926R (5ʹ-CCGYCAATTYMTTTRAG TTT-3ʹ).
  2. PCR products were pooled and purified using a DNA gel extraction kit (Axygen, Hangzhou, China). The DNA concentration was determined using a Quant-iT PicoGreen double-stranded DNA assay (Invitrogen, Germany), and quality was examined with a TBS-380 Mini-Fluorometer (Turner Biosystems, Sunnyvale, CA, USA). Finally, amplicons of all samples were pooled in equimolar concentrations and sequenced on an Illumina MiSeq2000 platform.

Bibliographic Citations

  1. Luo, W., Ding, H., Li, H., Ji, Z., Huang, K., Zhao, W., ... & Zeng, Y. (2020). Molecular diversity of the microbial community in coloured snow from the Fildes Peninsula (King George Island, Maritime Antarctica). Polar Biology, 43(9), 1391-1405.